Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E2F11

Cat no : 66089-1-Ig

Synonyms

Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor



Tested Applications

Positive WB detected inK562 cells, K-562 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66089-1-Ig is Factor XII Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XII fusion protein Ag3401
Full Name coagulation factor XII (Hageman factor)
Calculated molecular weight 615 aa, 68 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC012390
Gene symbol F12
Gene ID (NCBI) 2161
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Protocols

Product Specific Protocols
WB protocol for Factor XII antibody 66089-1-IgDownload protocol
IHC protocol for Factor XII antibody 66089-1-IgDownload protocol
IF protocol for Factor XII antibody 66089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li