CoraLite® Plus 488-conjugated Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E2F11

Cat no : CL488-66089

Synonyms

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66089

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66089 (Factor XII antibody) at dilution of 1:100.

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  IF Staining of mouse liver using CL488-66089

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Factor XII antibody (CL488-66089, Clone: 2E2F11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells, mouse liver tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-66089 targets Factor XII in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor XII fusion protein Ag3401
• Full Name: coagulation factor XII (Hageman factor)
• Calculated molecular weight: 615 aa, 68 kDa
• Observed molecular weight: 28 kDa
• GenBank accession number: BC012390
• Gene symbol: F12
• Gene ID (NCBI): 2161
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.