CoraLite® Plus 488-conjugated Factor XII Monoclonal antibody
Factor XII Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E2F11
Cat no : CL488-66089
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells, mouse liver tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66089 targets Factor XII in IF applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Factor XII fusion protein Ag3401 |
Full Name | coagulation factor XII (Hageman factor) |
Calculated molecular weight | 615 aa, 68 kDa |
Observed molecular weight | 28 kDa |
GenBank accession number | BC012390 |
Gene symbol | F12 |
Gene ID (NCBI) | 2161 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Factor XII antibody CL488-66089 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |