CoraLite®594-conjugated FAAH Monoclonal antibody

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  IF Staining of LNCaP using CL594-67607

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using CoraLite®594 FAAH antibody (CL594-67607, Clone: 3C5B5 ) at dilution of 1:200.

FAAH Monoclonal Antibody for IF/ICC

Cat No. CL594-67607

Clone No.3C5B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, rabbit, pig, mouse

Applications

IF/ICC

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

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Tested Applications

• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67607 targets FAAH in IF/ICC applications and shows reactivity with Human, rat, rabbit, pig, mouse samples.

• Tested Reactivity: Human, rat, rabbit, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12720

  Product name: Recombinant human FAAH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 236-579 aa of BC093632

  Sequence: TDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

• Full Name: fatty acid amide hydrolase
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC093632
• Gene Symbol: FAAH
• Gene ID (NCBI): 2166
• RRID: AB_3084778
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00519
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.