HRP-conjugated FC of Human IgG Monoclonal antibody

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  WB analysis using HRP-68275

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-68275 (FC of Human IgG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

FC of Human IgG Monoclonal Antibody for WB

Cat No. HRP-68275

Clone No.1A12C11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

anti-human IgG Fc, Fc Tag, hFc Tag, Human IgG, IgG Fc

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-68275 targets FC of Human IgG in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0212

  Product name: recombinant human FC of Human IgG protein

  Source: mammalian cells-derived, pINFUSE-hIgG2-Fc

  Tag: hIgG2-Fc

  Domain: 10004343 of

  Sequence:

• Full Name: FC of Human IgG
• Observed Molecular Weight: 55 kDa
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 10-20% of plasma protein. IgG FC is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. This antibody detects the human IgG FC heavy chain.