Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HepG2 cells, PC-3 cells, HK-2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A431 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82957-1-RR targets FDX1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3301 Product name: Recombinant human FDX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC017063 Sequence: MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS Predict reactive species |
| Full Name | ferredoxin 1 |
| Calculated Molecular Weight | 184 aa, 19 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC017063 |
| Gene Symbol | FDX1 |
| Gene ID (NCBI) | 2230 |
| RRID | AB_3670700 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FDX1 antibody 82957-1-RR | Download protocol |
| IF protocol for FDX1 antibody 82957-1-RR | Download protocol |
| IHC protocol for FDX1 antibody 82957-1-RR | Download protocol |
| WB protocol for FDX1 antibody 82957-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FDX1 Recombinant monoclonal antibody (catalog no. 82957-1-RR) has 1 total citation. It appears in Genes Chromosomes Cancer (PMID 42717274), a study investigating how IRF8 suppresses hepatocellular carcinoma progression through LIAS-dependent interferon production and cuproptosis activation. The associated research field is cancer biology, specifically liver cancer and cell death mechanisms.
| Species | Application | Title |
|---|---|---|
Genes Chromosomes Cancer IRF8 Suppresses Hepatocellular Carcinoma Progression Through LIAS-Dependent IFN Production and Cuproptosis Activation. |
