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- FITC Recombinant antibody, PBS Only

FITC Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

FITC conjugated

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2

Cat no : 80003-1-PBS

Synonyms

5-Isothiocyanat Fluorescein, FITC, Fluorescein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using - FITC Recombinant antibody (80003-1-RR)

    WB analysis using 80003-1-RR (same clone as 80003-1-PBS)

    FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80003-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80003-1-PBS targets FITC in WB, Indirect ELISA applications and shows reactivity with FITC conjugated samples.

Tested Reactivity FITC conjugated
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Compound
Full Name FITC
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.