FITC Recombinant antibody

FITC Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

FITC conjugated and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B2

Cat no : 80003-1-RR

Synonyms

5-Isothiocyanat Fluorescein, FITC, Fluorescein



Tested Applications

Positive WB detected inFITC conjugated antbody

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

80003-1-RR targets FITC in WB, IP, ELISA applications and shows reactivity with FITC conjugated samples.

Tested Reactivity FITC conjugated
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Compound
Full Name FITC
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882935
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Protocols

Product Specific Protocols
WB protocol for FITC antibody 80003-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Biomed Pharmacother

Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma

Authors - Jialiang Luo