FITC Recombinant monoclonal antibody

• 

  WB analysis using 80003-1-RR

  FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

FITC Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 80003-1-RR

Clone No.1B2

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

fitc conjugated and More (1)

Applications

WB, IF, IP, ELISA

Synonyms

5-Isothiocyanat Fluorescein, Fluorescein

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Tested Applications

• Positive WB detected in: FITC conjugated antbody

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• IP: See 1 publications below

Product Information

80003-1-RR targets FITC in WB, IF, IP, ELISA applications and shows reactivity with fitc conjugated samples.

• Tested Reactivity: fitc conjugated
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: FITC
• RRID: AB_2882935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Drug Dev Res; Carnosol alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats; Authors - Qing Li
human	IP	Biomed Pharmacother; Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma; Authors - Jialiang Luo