FKBP10/FKBP65 Polyclonal antibody, PBS Only

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• FKBP10/FKBP65 Polyclonal antibody, PBS Only (12172-1-PBS)

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  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12172-1-AP (same clone as 12172-1-PBS)

  WB result of FKBP10/FKBP65 antibody (12172-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12172-1-AP (same clone as 12172-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 12172-1-AP (same clone as 12172-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12172-1-AP (same clone as 12172-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12172-1-AP (same clone as 12172-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12172-1-AP (same clone as 12172-1-PBS)

  IP result of anti-FKBP10/FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12172-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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Product Information

12172-1-PBS targets FKBP10/FKBP65 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2814

  Product name: Recombinant human FKBP10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-582 aa of BC016467

  Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL

• Full Name: FK506 binding protein 10, 65 kDa
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC016467
• Gene Symbol: FKBP10
• Gene ID (NCBI): 60681
• RRID: AB_2102550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AY3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.