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  • KD/KO Validated

FMR1 Monoclonal antibody

FMR1 Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5B5

Cat no : 66548-1-Ig

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66548-1-Ig targets FMR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FMR1 fusion protein Ag4697
Full Name fragile X mental retardation 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 69-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_2881910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
WB protocol for FMR1 antibody 66548-1-IgDownload protocol
IHC protocol for FMR1 antibody 66548-1-IgDownload protocol
IP protocol for FMR1 antibody 66548-1-IgDownload protocol
FC protocol for FMR1 antibody 66548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.

Authors - Raeann Goering
  • KO Validated
mouseWB

Environ Res

Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease

Authors - Belinda J Petri
mouseIF

Front Mol Neurosci

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein

Authors - Cheng Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lisa (Verified Customer) (10-19-2021)

The antibody works quite well in WB, even if it is not possible to reuse the same diluted aliquot more than twice. I was not really satisfied of the results in IF, as the signal had a very high background.

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: hiPSCs-derived neurons