Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66548-1-Ig targets FMRP in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4697 Product name: Recombinant human FMR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC038998 Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI Predict reactive species |
| Full Name | fragile X mental retardation 1 |
| Calculated Molecular Weight | 632 aa, 71 kDa |
| Observed Molecular Weight | 69-72 kDa |
| GenBank Accession Number | BC038998 |
| Gene Symbol | FMR1 |
| Gene ID (NCBI) | 2332 |
| RRID | AB_2881910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FMRP antibody 66548-1-Ig | Download protocol |
| IHC protocol for FMRP antibody 66548-1-Ig | Download protocol |
| IP protocol for FMRP antibody 66548-1-Ig | Download protocol |
| WB protocol for FMRP antibody 66548-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FMRP Monoclonal antibody (2B5B5), catalog number 66548-1-Ig, has 5 total citations. These appear in Molecular Cell, Environmental Research, Frontiers in Molecular Neuroscience, Cell Biochemistry and Biophysics, and eLife. Research fields span viral RNA replication, hepatic m6A mRNA methylation in environmental liver disease, peripheral hypersensitivity and pain signaling, bladder cancer via METTL14/FMR1 pathway, and RNA localization in neuronal projections.
| Species | Application | Title |
|---|---|---|
Mol Cell Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication. | ||
Environ Res Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease | ||
Front Mol Neurosci Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein | ||
Cell Biochem Biophys Bladder Cancer Growth is Inhibited by Upregulating CircFUT8 through the METTL14/FMR1 Signaling Pathway | ||
Elife FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.
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Reviews
What customers say
The reviewer (Lisa Pavinato, Oct 2021) rated this antibody 3 out of 5 stars after using it for Western Blot and Immunofluorescence on hiPSCs-derived neurons. She found it works quite well in WB, though the diluted aliquot could not be reused more than twice. However, she was not satisfied with the IF results, reporting very high background signal. Overall, the antibody performs adequately for WB but may require optimization for IF applications to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (10-19-2021) | The antibody works quite well in WB, even if it is not possible to reuse the same diluted aliquot more than twice. I was not really satisfied of the results in IF, as the signal had a very high background.
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