FOXO4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86236-1-RR (same clone as 86236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86236-1-RR (FOXO4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86236-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 86236-1-RR (same clone as 86236-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using FOXO4 antibody (86236-1-RR, Clone: 250921F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86236-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86236-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86236-1-RR against Human FOXO4 were performed. The affinity constant is 2.40 nM.

FOXO4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86236-1-PBS

Clone No.250921F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AFX, AFX1, MLLT7

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Product Information

86236-1-PBS targets FOXO4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16169

  Product name: Recombinant human FOXO4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 198-505 aa of BC106761

  Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP

• Full Name: forkhead box O4
• Calculated Molecular Weight: 505 aa, 54 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC106761
• Gene Symbol: FOXO4
• Gene ID (NCBI): 4303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.