FOXO4 Recombinant monoclonal antibody

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  WB analysis using 86236-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86236-1-RR (FOXO4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293T using 86236-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using FOXO4 antibody (86236-1-RR, Clone: 250921F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86236-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86236-1-RR against Human FOXO4 were performed. The affinity constant is 2.40 nM.

FOXO4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86236-1-RR

Clone No.250921F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AFX, AFX1, MLLT7

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293T cells, HuT 78 cells, Ramos cells, Jurkat cells, PC-12 cells, mouse heart tissue
• Positive IF/ICC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86236-1-RR targets FOXO4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16169

  Product name: Recombinant human FOXO4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 198-505 aa of BC106761

  Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP

• Full Name: forkhead box O4
• Calculated Molecular Weight: 505 aa, 54 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC106761
• Gene Symbol: FOXO4
• Gene ID (NCBI): 4303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98177
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.