FSHB Monoclonal antibody

FSHB Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

1B10A11

Cat no : 67131-1-Ig

Synonyms

Follitropin beta chain, Follitropin subunit beta, FSH B, FSH beta, FSHB

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Validation Data Gallery

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  IHC staining of human pituitary using 67131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary using 67131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 67131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67131-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHB antibody (67131-1-Ig, Clone: 1B10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human pituitary tissue, human pituitary adenoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67131-1-Ig targets FSHB in IHC, IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FSHB fusion protein Ag13788
• Full Name: follicle stimulating hormone, beta polypeptide
• Calculated Molecular Weight: 129 aa, 15 kDa
• GenBank Accession Number: BC113488
• Gene Symbol: FSHB
• Gene ID (NCBI): 2488
• RRID: AB_2882430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).