FTCD Monoclonal antibody, PBS Only

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  WB analysis of mouse liver using 66979-1-Ig (same clone as 66979-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 66979-1-Ig (same clone as 66979-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66979-1-Ig (same clone as 66979-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66979-1-Ig (same clone as 66979-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 66979-1-Ig (same clone as 66979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FTCD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66979-1-PBS

Clone No.1H8A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formimidoyltetrahydrofolate cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Glutamate formimidoyltransferase, Glutamate formiminotransferase

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Product Information

66979-1-PBS targets FTCD in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FTCD fusion protein Ag17429
• Full Name: formiminotransferase cyclodeaminase
• Calculated Molecular Weight: 541 aa, 59 kDa
• Observed Molecular Weight: 53-62 kDa
• GenBank Accession Number: BC136395
• Gene Symbol: FTCD
• Gene ID (NCBI): 10841
• RRID: AB_2882299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95954
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine. This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).