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FUCA2 Polyclonal antibody, PBS Only (15157-1-PBS)

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WB analysis of human heart using 15157-1-AP (same clone as 15157-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
WB analysis using 15157-1-AP (same clone as 15157-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IP experiment of mouse heart using 15157-1-AP (same clone as 15157-1-PBS)
IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug. This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15157-1-AP (same clone as 15157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15157-1-PBS in a different storage buffer formulation.

FUCA2 Polyclonal antibody, PBS Only

Name: FUCA2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15157-1-PBS

FUCA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15157-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15157-1-PBS targets FUCA2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7250 Product name: Recombinant human FUCA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-467 aa of BC003060 Sequence: SGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKRDIVKELEVAIRNRTDLRFGLYYSLFEWFHPLFLEDESSSFHKRQFPVSKTLPELYELVNNYQPEVLWSDGDGGAPDQYWNSTGFLAWLYNESPVRGTVVTNDRWGAGSICKHGGFYTCSDRYNPGHLLPHKWENCMTIDKLSWGYRREAGISDYLTIEELVKQLVETVSCGGNLLMNIGPTLDGTISVVFEERLRQVGSWLKVNGEAIYETYTWRSQNDTVTPDVWYTSKPKEKLVYAIFLKWPTSGQLFLGHPKAILGATEVKLLGHGQPLNWISLEQNGIMVELPQLTIHQMPCKWGWALALTNVI Predict reactive species
Full Name	fucosidase, alpha-L- 2, plasma
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	54-60 kDa
GenBank Accession Number	BC003060
Gene Symbol	FUCA2
Gene ID (NCBI)	2519
RRID	AB_2104956
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BTY2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.