FYN Monoclonal antibody

• Featured Product
• KD/KO Validated

FYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

1G4B1

Reviews (1)

Cat no : 66606-1-Ig

Synonyms

FYN, p59 Fyn, Proto oncogene c Fyn, Proto oncogene Syn, SLK, Src like kinase, SYN, Tyrosine protein kinase Fyn

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Validation Data Gallery

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  WB analysis of HEK-293 using 66606-1-Ig

  WB result of FYN antibody (66606-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYN transfected HEK-293 cells.

• 

  WB analysis using 66606-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66606-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66606-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66606-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66606-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66606-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66606-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66606-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66606-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 66606-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody (66606-1-Ig, Clone: 1G4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, Ramos cells, HeLa cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochemistry (IHC): IHC : 1:2000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 3 publications below

Product Information

66606-1-Ig targets FYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FYN fusion protein Ag11127
• Full Name: FYN oncogene related to SRC, FGR, YES
• Calculated Molecular Weight: 537aa,61 kDa; 482aa,55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032496
• Gene Symbol: FYN
• Gene ID (NCBI): 2534
• RRID: AB_2881966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

Publications

Species	Application	Title
mouse	WB	EBioMedicine; Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.; Authors - Xiaoxiao Luo
human,mouse	IHC	EBioMedicine; Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia.; Authors - Maya Barad
human	WB	Biochem Pharmacol; PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3.; Authors - Mingming Sun
human	IHC	Front Oncol; A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma; Authors - Hao Wang
human	WB	Exp Cell Res; ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.; Authors - Hongyu Yuan
human	WB	Exp Cell Res; Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.; Authors - Ting Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (07-11-2022)

A reliable antibody in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

FYN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66606-1-Ig)