CoraLite® Plus 488-conjugated FYN Monoclonal antibody

• Featured Product
• KD/KO Validated

FYN Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

1G4B1

Cat no : CL488-66606

Synonyms

Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66606

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66606 (FYN antibody) at dilution of 1:50.

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  IF Staining of Neuro-2a using CL488-66606

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite® Plus 488 FYN antibody (CL488-66606, Clone: 1G4B1 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66606

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human FYN (CL488-66606, Clone:1G4B1) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: Neuro-2a cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL488-66606 targets FYN in WB, IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FYN fusion protein Ag11127
• Full Name: FYN oncogene related to SRC, FGR, YES
• Calculated Molecular Weight: 537aa,61 kDa; 482aa,55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032496
• Gene Symbol: FYN
• Gene ID (NCBI): 2534
• RRID: AB_2883360
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu