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  • KD/KO Validated

FZR1/Cdh1 Polyclonal antibody

FZR1/Cdh1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16368-1-AP

Synonyms

CDC20 like protein 1, CDC20C, CDH1, Cdh1/Hct1 homolog, Fizzy related protein homolog, FYR, FZR, FZR1, FZR2, HCDH, HCDH1, KIAA1242



Tested Applications

Positive WB detected inHeLa cells, C6 cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16368-1-AP targets FZR1/Cdh1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FZR1/Cdh1 fusion protein Ag9201
Full Name fizzy/cell division cycle 20 related 1 (Drosophila)
Calculated molecular weight 493 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC013413
Gene symbol FZR1
Gene ID (NCBI) 51343
RRIDAB_2107380
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.

Protocols

Product Specific Protocols
WB protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IHC protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IF protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IP protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
mouseWB

Sci Adv

The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes.

Authors - Changyin Zhou
  • KD Validated
humanWB

Acta Pharmacol Sin

Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis.

Authors - Ling Yang
mouseWB

Mol Cell Biol

ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction.

Authors - Marie-Claude Perry
IP,WB

iScience

Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Authors - Arielle N Valdez-Sinon
  • KD Validated
mouseFC

Cell Death Dis

A Cdh1-FoxM1-Apc axis controls muscle development and regeneration.

Authors - Zhe Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (09-07-2020)

This antibody appears to be good for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HaCaT and HEK293 cells