|Positive WB detected in||HeLa cells, C6 cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16368-1-AP targets FZR1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FZR1 fusion protein Ag9201|
|Full Name||fizzy/cell division cycle 20 related 1 (Drosophila)|
|Calculated molecular weight||493 aa, 55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC013413|
|Gene ID (NCBI)||51343|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.
|Product Specific Protocols|
|WB protocol for FZR1 antibody 16368-1-AP||Download protocol|
|IHC protocol for FZR1 antibody 16368-1-AP||Download protocol|
|IF protocol for FZR1 antibody 16368-1-AP||Download protocol|
|IP protocol for FZR1 antibody 16368-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chun (Verified Customer) (09-07-2020)
This antibody appears to be good for immunoblotting.