Validation Data Gallery
|Positive WB detected in
|HeLa cells, C6 cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells
|Positive IP detected in
|mouse heart tissue
|Positive IHC detected in
|human liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16368-1-AP targets FZR1/Cdh1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|FZR1/Cdh1 fusion protein Ag9201
|fizzy/cell division cycle 20 related 1 (Drosophila)
|Calculated molecular weight
|493 aa, 55 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.
|Product Specific Protocols
|WB protocol for FZR1/Cdh1 antibody 16368-1-AP
|IHC protocol for FZR1/Cdh1 antibody 16368-1-AP
|IF protocol for FZR1/Cdh1 antibody 16368-1-AP
|IP protocol for FZR1/Cdh1 antibody 16368-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chun (Verified Customer) (09-07-2020)
This antibody appears to be good for immunoblotting.