Factor XIIIa Monoclonal antibody

Factor XIIIa Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 66325-1-Ig

Synonyms

Coagulation factor XIIIa, F13A, F13A1, Factor XIIIA, Transglutaminase A chain



Tested Applications

Positive WB detected inhuman placenta tissue, mouse liver tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:800
Sample-dependent, check data in validation data gallery

Product Information

66325-1-Ig targets Factor XIIIa in WB, IHC, FC,ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XIIIa fusion protein Ag11105
Full Name coagulation factor XIII, A1 polypeptide
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC027963
Gene symbol F13A1
Gene ID (NCBI) 2162
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

Protocols

Product Specific Protocols
WB protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
IHC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
FC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols