FcZero-rAb® Mouse Activated T Cell Panel

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  1x10^6 anti-mouse CD3/CD28 stimulated mouse splenocytes were stained with PK30059 FcZero-rAb® Mouse Activated T Cell Panel. CD3+ cells are gated. Parent population: live cells. Cells were not fixed.

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  1x10^6 anti-mouse CD3/CD28 stimulated mouse splenocytes were stained with PK30059 FcZero-rAb® Mouse Activated T Cell Panel. CD4 and CD8 expression on CD3+ cells is shown. Cells were not fixed.

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  1x10^6 anti-mouse CD3/CD28 stimulated mouse splenocytes were stained with PK30059 FcZero-rAb® Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD4+ T lymphocytes is shown. Cells were not fixed.

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  1x10^6 anti-mouse CD3/CD28 stimulated mouse splenocytes were stained with PK30059 FcZero-rAb® Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD8+ T lymphocytes is shown. Cells were not fixed.

Cat No. PK30059

Host

Rabbit

Reactivity

Mouse

Applications

FC

Conjugate

Various

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Product Information

T lymphocytes are immune cells that respond to foreign antigens by secreting cytokines (CD8⁺ Cytotoxic T  cells) and coordinating the immune response (CD4⁺ Helper T cells).

This panel is designed for the identification of CD3⁺ T lymphocytes into CD4⁺ T lymphocyte and CD8⁺T  lymphocyte subpopulations and the evaluation of these two populations for activation with CD69 and  CD25.

Components

Fluorochrome (ex/em)	Catalog No.	Size	Target	Clone	Isotype	Description
APC-Cyanine7 (650 nm/778 nm)	AY7-FcA65659	100 ug	CD3	17-A2	Rabbit IgG	T cell marker
CoraLite® Plus 647 (650 nm/660 nm)	CL647-FcA98288	100 ug	CD25	242494H5	Rabbit IgG	Marker of activation
CoraLite® Plus 488 (493 nm/522 nm)	CL488-FcA98440	100 ug	CD69	250269C11	Rabbit IgG	Marker of activation
CoraLite® Plus 405 (399 nm/422 nm)	CL405-FcA65655	100 ug	CD4	RM4-4	Rabbit IgG	Helper T cell marker
PE (496 nm, 565 nm/578 nm)	PE-FcA98472-2	100 ug	CD8	242879C10	Rabbit IgG	Cytotoxic T cell marker

5 antibodies (1 vial each), 100 ug per vial.

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Usage

The suggested use is 0.1-0.25 ug of each antibody per 10^6 cells in a 100 uL suspension or 0.1-0.25 ug of each antibody per 100 uL of whole blood.

Flow-Cytometry-Cell-Surface-Staining-Protocol

Cited in Article as

PK30059, FcZero-rAb® Mouse Activated T Cell Panel, Proteintech, IL, USA