CoraLite® Plus 488-conjugated Fibronectin Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated Fibronectin Recombinant monoclonal antibody (CL488-80911-4)

Fibronectin Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-80911-4

Clone No.251989E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

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  IF Staining of NIH/3T3 using CL488-80911-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Fibronectin antibody (CL488-80911-4, Clone: 251989E12 ) at dilution of 1:200.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80911-4 targets Fibronectin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5117

  Product name: Recombinant Human Fibronectin protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 607-1265 aa of NM_212482.4

  Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

• Full Name: fibronectin 1
• Calculated Molecular Weight: 272 kDa
• Observed Molecular Weight: 220-275 kDa
• GenBank Accession Number: NM_212482.4
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_3747295
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02751-15
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa.