Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 60223-1-Ig


14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1

Tested Applications

Positive WB detected inHepG2 cells, human heart tissue, human placenta tissue, L02 cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, human colon cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60223-1-Ig is Galectin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC020675
Gene symbol LGALS1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).


Product Specific Protocols
WB protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IHC protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IF protocol for Galectin-1 antibody 60223-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.

Authors - Maria Claudia Vladoiu

Sci Rep

Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.

Authors - Marilyne Labrie

Int J Oncol

Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

Authors - Qi Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guorong (Verified Customer) (05-23-2022)

It is excellent with ARPE-19 cells but gives no signal with 293T

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T and ARPE-19
Galectin-1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:60223-1-Ig)

Sushmita (Verified Customer) (12-16-2019)

This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Pancreatic cancer cell line
Galectin-1 Antibody Western Blot, validation (1:4000 dilution) in Pancreatic cancer cell line (Cat no:60223-1-Ig)