Validation Data Gallery
|Positive WB detected in||HCT 116 cells, HepG2 cells, human placenta tissue, human heart tissue, L02 cells, U2OS cells, A549 cells, HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U2OS cells, Saos-2 cells, human colon cancer tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
60223-1-Ig targets Galectin-1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||Galectin-1 fusion protein Ag2432|
|Full Name||lectin, galactoside-binding, soluble, 1|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||13 kDa|
|GenBank accession number||BC020675|
|Gene ID (NCBI)||3956|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy
Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.
Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.
Int J Oncol
Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (05-23-2022)
It is excellent with ARPE-19 cells but gives no signal with 293T
Sushmita (Verified Customer) (12-16-2019)
This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.