|Positive WB detected in||HepG2 cells, human heart tissue, human placenta tissue, L02 cells, U2OS cells|
|Positive IHC detected in||human colon cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U2OS cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60223-1-Ig is Galectin-1 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG1|
|Immunogen||Galectin-1 fusion protein Ag2432|
|Full Name||lectin, galactoside-binding, soluble, 1|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||13 kDa|
|GenBank accession number||BC020675|
|Gene ID (NCBI)||3956|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.
Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sushmita (Verified Customer) (12-16-2019)
This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.