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CoraLite®594-conjugated GAPDH (Human Specific) Recombinant antibody

GAPDH (Human Specific) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G21

Cat no : CL594-80570

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated GAPDH (Human Specific) Rec (CL594-80570)

    FC experiment of HeLa using CL594-80570

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GAPDH (Human Specific) (CL594-80570, Clone:2G21) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80570 targets GAPDH (Human Specific) in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAPDH (Human Specific) fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Protocols

Product Specific Protocols
FC protocol for CL594 GAPDH (Human Specific) antibody CL594-80570Download protocol
Standard Protocols
Click here to view our Standard Protocols