CoraLite®594-conjugated GARS Monoclonal antibody

GARS Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H9D3

Cat no : CL594-67893

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

---

Validation Data Gallery

• 

  IF Staining of A431 using CL594-67893

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 GARS antibody (CL594-67893, Clone: 2H9D3 ) at dilution of 1:200.

• 

  FC experiment of A431 using CL594-67893

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GARS (CL594-67893, Clone:2H9D3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF detected in: A431 cells
• Positive FC detected in: A431 cells, A431 cell line

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67893 targets GARS in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GARS fusion protein Ag8432
• Full Name: glycyl-tRNA synthetase
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC007755
• Gene Symbol: GARS
• Gene ID (NCBI): 2617
• RRID: AB_2920188
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.