GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24472-1-AP

Synonyms

ARHGEF2, GEF, GEF H1, GEFH1, GEF-H1, KIAA0651, LFP40, Microtubule regulated Rho GEF, P40

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Validation Data Gallery

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  WB analysis using 24472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24472-1-AP (GEF-H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24472-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24472-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24472-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 24472-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 24472-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 24472-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 24472-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 24472-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human GEF-H1 (24472-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, HeLa cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, MCF-7 cells, HeLa cells
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

24472-1-AP targets GEF-H1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEF-H1 fusion protein Ag13352
• Full Name: rho/rac guanine nucleotide exchange factor (GEF) 2
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC020567
• Gene Symbol: ARHGEF2
• Gene ID (NCBI): 9181
• RRID: AB_2879560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho