GFM1 Polyclonal antibody, PBS Only

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• GFM1 Polyclonal antibody, PBS Only (14274-1-PBS)

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  WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

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Product Information

14274-1-PBS targets GFM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5616

  Product name: Recombinant human GFM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 400-751 aa of BC049210

  Sequence: RMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN

• Full Name: G elongation factor, mitochondrial 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC049210
• Gene Symbol: GFM1
• Gene ID (NCBI): 85476
• RRID: AB_2110140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RP9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]