GIRK2 Recombinant antibody

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  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86016-1-RR against Human GIRK2 were performed. The affinity constant is 0.372 nM.

GIRK2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86016-1-RR

Clone No.250605D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KCNJ6, BIR1, G protein-activated inward rectifier potassium channel 2, GIRK 2, GIRK-2

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Tested Applications

• Positive WB detected in: MG-63 cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86016-1-RR targets GIRK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GIRK2 fusion protein Ag16344
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 6
• Calculated Molecular Weight: 423 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC101547
• Gene Symbol: GIRK2
• Gene ID (NCBI): 3763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48051
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIRK2 (also known as Kir3.2), encoded by KCNJ6 gene, is a member of the G protein-coupled inwardly-rectifying potassium channel (GIRK, Kir3) family of inward rectifier potassium channels. GIRK channels are activated following stimulation of G protein-coupled receptors (PMID: 26422984). They play important roles in regulating cellular excitabilities in the heart and brain (PMID: 31043612). Mutations in KCNJ6 gene are associated with Keppen-Lubinsky Syndrome (PMID: 25620207).