GLI2-Specific Polyclonal antibody, PBS Only

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• GLI2-Specific Polyclonal antibody, PBS Only (18989-1-PBS)

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  WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 18989-1-AP (same clone as 18989-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 18989-1-AP (same clone as 18989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GLI2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

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Product Information

18989-1-PBS targets GLI2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GLI family zinc finger 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 167 kDa， 88 kDa
• GenBank Accession Number: NM_005270
• Gene Symbol: GLI2
• Gene ID (NCBI): 14633
• RRID: AB_10596479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10070
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.