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GLRB Polyclonal antibody, PBS Only (15371-1-PBS)

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WB analysis of mouse brain using 15371-1-AP (same clone as 15371-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 15371-1-AP (same clone as 15371-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
WB analysis of mouse spinal cord using 15371-1-AP (same clone as 15371-1-PBS)
mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15371-1-AP (same clone as 15371-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15371-1-PBS in a different storage buffer formulation.

GLRB Polyclonal antibody, PBS Only

Name: GLRB Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15371-1-PBS

GLRB Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15371-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15371-1-PBS targets GLRB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4429 Product name: Recombinant human GLRB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-267 aa of BC032635 Sequence: VEEAYSKEKSSKKGKGKKKQYLCPSQQSAEDLARVPANSTSNILNRLLVSYDPRIRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWKPDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVG Predict reactive species
Full Name	glycine receptor, beta
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	56 kDa
GenBank Accession Number	BC032635
Gene Symbol	GLRB
Gene ID (NCBI)	2743
RRID	AB_2878132
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48167
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)