golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (2)

Cat no : 12640-1-AP

Synonyms

GOLGA1, Golgi marker, golgin 97, Golgin subfamily A member 1

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Validation Data Gallery

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  WB analysis using 12640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 12640-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 12640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 12640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12640-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human golgin 97 (12640-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

12640-1-AP targets golgin 97 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: golgin 97 fusion protein Ag3335
• Full Name: golgi autoantigen, golgin subfamily a, 1
• Calculated Molecular Weight: 767 aa, 88 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC032853
• Gene Symbol: GOLGA1
• Gene ID (NCBI): 2800
• RRID: AB_2115315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.; Authors - Lucía Barbier-Torres
human,mouse	IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Dev Cell; GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.; Authors - Xiaoyan Zhang
human	WB	Cell Rep; CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth; Authors - Shasha Yin
human	IF	Cell Chem Biol; Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.; Authors - Nan Qiu
human	WB,IF	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-17-2023)

very good for WB

• Applications: Western Blot

FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

• Applications: Western Blot