golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12640-1-AP

Synonyms

GOLGA1, Golgi marker, golgin 97, Golgin subfamily A member 1



Tested Applications

Positive WB detected inHEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12640-1-AP targets golgin 97 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen golgin 97 fusion protein Ag3335
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol GOLGA1
Gene ID (NCBI) 2800
RRIDAB_2115315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
WB protocol for golgin 97 antibody 12640-1-APDownload protocol
IF protocol for golgin 97 antibody 12640-1-APDownload protocol
FC protocol for golgin 97 antibody 12640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF,WB

Nat Commun

TBC1D23 mediates Golgi-specific LKB1 signaling

Authors - Yingfeng Tu
mouseIF

Nat Commun

Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.

Authors - Lucía Barbier-Torres
human,mouseIF

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
humanWB

Dev Cell

GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.

Authors - Xiaoyan Zhang
humanWB

Cell Rep

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

Authors - Shasha Yin
humanIF

Cell Chem Biol

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Authors - Nan Qiu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-17-2023)

very good for WB

  • Applications: Western Blot
FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

  • Applications: Western Blot