Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, SMMC-7721 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12640-1-AP targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3335 Product name: Recombinant human GOLGA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 418-767 aa of BC032853 Sequence: QIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 1 |
| Calculated Molecular Weight | 767 aa, 88 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC032853 |
| Gene Symbol | golgin 97 |
| Gene ID (NCBI) | 2800 |
| RRID | AB_2115315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92805 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for golgin 97 antibody 12640-1-AP | Download protocol |
| IF protocol for golgin 97 antibody 12640-1-AP | Download protocol |
| WB protocol for golgin 97 antibody 12640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Golgin 97 Polyclonal antibody (catalog #12640-1-AP) has 44 total citations. These appear in high-impact journals including Nature, Cell, Nature Communications, Cell Reports, Cell Discovery, Developmental Cell, Molecular Metabolism, EMBO Reports, Journal of Biological Chemistry, Structure, and Methods in Molecular Biology, among others. Research fields span Golgi apparatus structure and dynamics, intracellular trafficking and vesicle transport, autophagy, endosomal complexes, STING signaling, pancreatic β-cell function, cancer biology, and neurodegenerative disease.
| Species | Application | Title |
|---|---|---|
Nat Commun Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease. | ||
Nat Commun Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy. | ||
Nat Commun TUG protein acts through a disordered region to organize the early secretory pathway. |
Reviews
What customers say
Both reviews rate this antibody 5 out of 5 stars for Western Blot. Users consistently describe it as "very good for WB," indicating strong performance and satisfaction with its specificity and signal quality in this application. No negative feedback or issues were reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (04-17-2023) | very good for WB
|
Boyan (Verified Customer) (04-02-2023) | very good for WB.
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