GP73/GOLPH2 Monoclonal antibody

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  WB analysis using 66331-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66331-1-Ig (GP73/GOLPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66331-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66331-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66331-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (66331-1-Ig, Clone: 3D1F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

GP73/GOLPH2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66331-1-Ig

Clone No.3D1F1

Publications(5)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C9orf155, Golgi marker, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, GP73/GOLPH2

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

66331-1-Ig targets GP73/GOLPH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Full Name: golgi membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC001740
• Gene Symbol: GP73
• Gene ID (NCBI): 51280
• RRID: AB_2881712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NBJ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

Publications

Species	Application	Title
human	WB	Front Oncol; TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.; Authors - Meng Wang
human	WB	Cell Rep; Cholesterol suppresses GOLM1-dependent selective autophagy of RTKs in hepatocellular carcinoma.; Authors - Wei-Qing Shao ; KD Validated
human	WB	Microbiol Spectr; Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-γ1(S1248); Authors - Chang Liu
mouse	WB	J Transl Med; PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1; Authors - Fuqiang Huang
	IF	Histochem Cell Biol; Expression of SARS-CoV-2 entry molecules ACE2, NRP1, TMPRSS2, and FURIN in the reproductive tissues of male macaques; Authors - Ryutaro Moriyama