GPR101-Specific Polyclonal antibody

GPR101-Specific Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20074-1-AP

Synonyms

Probable G-protein coupled receptor 101, GPR101

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Validation Data Gallery

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  WB analysis of HEK-293 using 20074-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 20074-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 20074-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, COS-7 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20074-1-AP targets GPR101-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 101
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_054021
• Gene Symbol: GPR101
• Gene ID (NCBI): 83550
• RRID: AB_10665356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.