GPR101-Specific Polyclonal antibody, PBS Only

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• GPR101-Specific Polyclonal antibody, PBS Only (20074-1-PBS)

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  WB analysis of HEK-293 using 20074-1-AP (same clone as 20074-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 20074-1-AP (same clone as 20074-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20074-1-AP (same clone as 20074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20074-1-AP (same clone as 20074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 20074-1-AP (same clone as 20074-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20074-1-PBS in a different storage buffer formulation.

GPR101-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 20074-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

GPR101, Probable G-protein coupled receptor 101

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Product Information

20074-1-PBS targets GPR101-Specific in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: G protein-coupled receptor 101
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_054021
• Gene Symbol: GPR101
• Gene ID (NCBI): 83550
• RRID: AB_10665356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96P66
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.