GPR22 Polyclonal antibody

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• GPR22 Polyclonal antibody (19951-1-AP)

GPR22 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19951-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

G protein coupled receptor 22, G-protein coupled receptor 22

• 

  WB analysis of mouse heart using 19951-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 19951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 19951-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of SH-SY5Y using 19951-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug GPR22 Polyclonal antibody (19951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19951-1-AP targets GPR22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: G protein-coupled receptor 22
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_005295
• Gene Symbol: GPR22
• Gene ID (NCBI): 2845
• RRID: AB_10644129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99680
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR22 Belongs to the G-protein coupled receptor 1 family. GPR22 is an Orphan receptor. The antibody recognize the C-term of GPR22.

Publications

What published studies show

The GPR22 Polyclonal antibody (Cat# 19951-1-AP) has 1 total citation. It was cited in BMC Cardiovascular Disorders (2024), in a study investigating cardiomyocyte-specific overexpression of GPR22 to ameliorate cardiac injury in mice with acute myocardial infarction. The research field is cardiovascular disease, specifically myocardial infarction and cardiac protection. The antibody was used for Western blot in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	BMC Cardiovasc Disord; Cardiomyocyte-specific overexpression of GPR22 ameliorates cardiac injury in mice with acute myocardial infarction.; Authors - Chin-Chuan Chang