GPRC5A,RAI3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPRC5A,RAI3 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10309-1-AP

Synonyms

GPCR5A, GPRC5A, GPRC5A,RAI3, RAI3, RAIG 1, RAIG1

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Validation Data Gallery

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  WB analysis using 10309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10309-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of MCF-7 using 10309-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug GPRC5A,RAI3 antibody (10309-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: A375 cells, MCF-7 cells, HeLa cells, RAW 264.7 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

10309-1-AP targets GPRC5A,RAI3 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPRC5A,RAI3 fusion protein Ag0311
• Full Name: G protein-coupled receptor, family C, group 5, member A
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC003665
• Gene Symbol: GPRC5A
• Gene ID (NCBI): 9052
• RRID: AB_2877732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Publications

Species	Application	Title
goat	IHC	Cell Prolif; Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis; Authors - Peng Lin
human	WB	Sci Total Environ; GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.; Authors - Ziling Huang ; KO Validated
human	WB	Oncol Lett; Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.; Authors - Er Jin
human	WB	Exp Lung Res; GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.; Authors - Jin Er
		Mol Med Rep; Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.; Authors - Er Jin
human	WB	Stem Cell Res Ther; TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties; Authors - Mingzhu Cao