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GPRC5A,RAI3 Polyclonal antibody

GPRC5A,RAI3 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 10309-1-AP

Synonyms

GPCR5A, GPRC5A, GPRC5A,RAI3, RAI3, RAIG 1, RAIG1



Tested Applications

Positive WB detected inA375 cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 10309-1-AP is GPRC5A,RAI3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPRC5A,RAI3 fusion protein Ag0311
Full Name G protein-coupled receptor, family C, group 5, member A
Calculated molecular weight 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC003665
Gene symbol GPRC5A
Gene ID (NCBI) 9052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Protocols

Product Specific Protocols
WB protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
IHC protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
FC protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.

Authors - Er Jin
humanWB

Exp Lung Res

GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.

Authors - Jin Er
humanWB

Oncol Lett

Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.

Authors - Er Jin
humanWB

Sci Total Environ

GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.

Authors - Ziling Huang
  • KO Validated