|Positive WB detected in||A375 cells, MCF-7 cells, RAW 264.7 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 10309-1-AP is GPRC5A,RAI3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPRC5A,RAI3 fusion protein Ag0311|
|Full Name||G protein-coupled receptor, family C, group 5, member A|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC003665|
|Gene ID (NCBI)||9052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )
Mol Med Rep
Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.
Exp Lung Res
GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.
Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.
Sci Total Environ
GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.