GRAMD1B Polyclonal antibody
GRAMD1B Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24905-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Positive FC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
24905-1-AP targets GRAMD1B in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GRAMD1B fusion protein Ag20670 |
Full Name | GRAM domain containing 1B |
Calculated Molecular Weight | 738 aa, 85 kDa |
Observed Molecular Weight | 85 kDa |
GenBank Accession Number | BC107480 |
Gene Symbol | GRAMD1B |
Gene ID (NCBI) | 57476 |
RRID | AB_2879791 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.
Protocols
Product Specific Protocols | |
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WB protocol for GRAMD1B antibody 24905-1-AP | Download protocol |
IHC protocol for GRAMD1B antibody 24905-1-AP | Download protocol |
IF protocol for GRAMD1B antibody 24905-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Lipid Res ASTER-B regulates mitochondrial carotenoid transport and homeostasis
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