GRIA1 Monoclonal antibody

GRIA1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1F4A1

Reviews (2)

Cat no : 67642-1-Ig

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

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Validation Data Gallery

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  WB analysis using 67642-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spinal cord using 67642-1-Ig

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67642-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67642-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, pig spinal cord tissue, pig cerebellum tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

67642-1-Ig targets GRIA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRIA1 fusion protein Ag28932
• Full Name: glutamate receptor, ionotropic, AMPA 1
• Calculated Molecular Weight: 906 aa, 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC111734
• Gene Symbol: GRIA1
• Gene ID (NCBI): 2890
• RRID: AB_2882842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
rat	WB	Exp Neurol; Long-term variable photoperiod exposure impairs the mPFC and induces anxiety and depression-like behavior in male wistar rats; Authors - YuanYuan Hou
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
rat	WB	J Psychiatr Res; Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats.; Authors - Dan Yao
mouse	WB	Cell Biosci; Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling; Authors - Xing-Yang Liu
mouse	WB,IF,IP	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pegah (Verified Customer) (02-03-2023)

I used a concentration of 1:500 of GRIA1 Monoclonal antibody (67642-1-Ig) for paraformaldehyde fixed mouse brain tissue with 40um diameter.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

GRIA1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain (Cat no:67642-1-Ig)

FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is very strong and clean. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons