GRIA1 Monoclonal antibody, PBS Only

• 

  WB analysis using 67642-1-Ig (same clone as 67642-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spinal cord using 67642-1-Ig (same clone as 67642-1-PBS)

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 67642-1-Ig (same clone as 67642-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67642-1-Ig (same clone as 67642-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

GRIA1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67642-1-PBS

Clone No.1F4A1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

---

Product Information

67642-1-PBS targets GRIA1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28932

  Product name: Recombinant human GRIA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 631-906 aa of BC111734

  Sequence: TANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

• Full Name: glutamate receptor, ionotropic, AMPA 1
• Calculated Molecular Weight: 906 aa, 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC111734
• Gene Symbol: GRIA1
• Gene ID (NCBI): 2890
• RRID: AB_2882842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42261
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.