CoraLite®594-conjugated GRIA1 Monoclonal antibody

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  IF Staining of mouse brain using CL594-67642

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GRIA1 antibody (CL594-67642, Clone: 1F4A1 ) at dilution of 1:200.

GRIA1 Monoclonal Antibody for IF-P

Cat No. CL594-67642

Clone No.1F4A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF-P

Synonyms

1F4A1, AMPA-selective glutamate receptor 1, GluA1, GLUH1, GluR 1

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67642 targets GRIA1 in IF-P applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28932

  Product name: Recombinant human GRIA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 631-906 aa of BC111734

  Sequence: TANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

• Full Name: glutamate receptor, ionotropic, AMPA 1
• Calculated Molecular Weight: 906 aa, 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC111734
• Gene Symbol: GRIA1
• Gene ID (NCBI): 2890
• RRID: AB_3084781
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42261
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.