CoraLite®594-conjugated GRIA1 Monoclonal antibody

GRIA1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F4A1

Cat no : CL594-67642

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67642

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GRIA1 antibody (CL594-67642, Clone: 1F4A1 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67642

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GRIA1 (CL594-67642, Clone:1F4A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse brain tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67642 targets GRIA1 in IF, FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRIA1 fusion protein Ag28932
• Full Name: glutamate receptor, ionotropic, AMPA 1
• Calculated Molecular Weight: 906 aa, 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC111734
• Gene Symbol: GRIA1
• Gene ID (NCBI): 2890
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.