CoraLite®594-conjugated GRP94 Monoclonal antibody

GRP94 Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G7C7

Cat no : CL594-60012

Synonyms

ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells
Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60012-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60012 targets GRP94 in WB, IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP94 fusion protein Ag1439
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 96 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC009195
Gene symbol HSP90B1
Gene ID (NCBI) 7184
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for CL594 GRP94 antibody CL594-60012Download protocol
IF protocol for CL594 GRP94 antibody CL594-60012Download protocol
Standard Protocols
Click here to view our Standard Protocols