|Positive WB detected in||A549 cells, pig brain tissue, HEK-293 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67329-1-Ig targets GSK3B in WB, IHC, IF,ELISA applications and shows reactivity with Human, Pig samples.
|Tested Reactivity||Human, Pig|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GSK3B fusion protein Ag17320|
|Full Name||glycogen synthase kinase 3 beta|
|Calculated molecular weight||433 aa, 48 kDa|
|Observed molecular weight||46-48 kDa|
|GenBank accession number||BC000251|
|Gene ID (NCBI)||2932|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .
Med Sci Monit
Protective Effects of Ischemic Postconditioning on Livers in Rats with Limb Ischemia-Reperfusion via Glycogen Synthase Kinase 3 beta (GSK-3β)/Fyn/Nuclear Receptor-Erythroid-2-Related Factor (Nrf2) Pathway.
J Inflamm Res
Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.