Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67272-1-Ig targets Granzyme K in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27297 Product name: Recombinant human GZMK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 85-215 aa of BC035802 Sequence: HSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSG Predict reactive species |
| Full Name | granzyme K (granzyme 3; NK tryptase II) |
| Calculated Molecular Weight | 29 kDa |
| GenBank Accession Number | BC035802 |
| Gene Symbol | GZMK |
| Gene ID (NCBI) | 3003 |
| RRID | AB_2882541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49863 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Granzyme K antibody 67272-1-Ig | Download protocol |
| IHC protocol for Granzyme K antibody 67272-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Granzyme K Monoclonal antibody (Cat# 67272-1-Ig) has 12 citations published in journals including Nature Communications, Neuron, Advanced Science, Med, Cancer Letters, Signal Transduction and Targeted Therapy, NPJ Precision Oncology, Cell Communication and Signaling, Journal of Inflammation Research, Biomedicines, and Frontiers in Immunology. Research fields span oncology (lung adenocarcinoma, renal cell carcinoma, acute myeloid leukemia), immunotherapy response, autoimmune diseases (Sjögren's syndrome, Hashimoto's thyroiditis, psoriasis), cardiac myxoma, and spinal cord wound healing.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Revealing the crucial roles of suppressive immune microenvironment in cardiac myxoma progression. | ||
Nat Commun Molecular and spatial analysis of tertiary lymphoid structures in Sjogren's syndrome | ||
Neuron Clonally expanded, targetable, natural killer-like NKG7 T cells seed the aged spinal cord to disrupt myeloid-dependent wound healing | ||
Adv Sci (Weinh) Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics | ||
Med A machine learning model integrating circulating Temra cell transcriptional profiles to predict immunotherapy efficacy. | ||
Cancer Lett A single-cell map of patients with non-small cell lung cancer harboring rare-driver mutations after anti-PD-1 treatment |
