Granzyme K Monoclonal antibody

Granzyme K Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse and More (1)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A4

Cat no : 67272-1-Ig

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67272-1-Ig targets Granzyme K in IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Granzyme K fusion protein Ag27297
Full Name granzyme K (granzyme 3; NK tryptase II)
Calculated molecular weight 29 kDa
GenBank accession numberBC035802
Gene symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2882541
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.

Protocols

Product Specific Protocols
IHC protocol for Granzyme K antibody 67272-1-IgDownload protocol
IF protocol for Granzyme K antibody 67272-1-IgDownload protocol
FC protocol for Granzyme K antibody 67272-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomedicines

Identification and Preliminary Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Hashimoto's Thyroiditis by Comprehensive Analysis

Authors - Zihan Xi
humanIF

Adv Sci (Weinh)

Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics

Authors - Xin Zhang