CoraLite® Plus 488-conjugated Granzyme K Monoclonal antibody

Granzyme K Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3A4

Cat no : CL488-67272

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-67272

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Granzyme K antibody (CL488-67272, Clone: 1C3A4 ) at dilution of 1:200. DAPI (blue).

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  FC experiment of NK92 using CL488-67272

  1X10^6 NK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Granzyme K (CL488-67272, Clone:1C3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: NK92, NK92 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67272 targets Granzyme K in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Granzyme K fusion protein Ag27297
• Full Name: granzyme K (granzyme 3; NK tryptase II)
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC035802
• Gene Symbol: GZMK
• Gene ID (NCBI): 3003
• RRID: AB_2919442
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.