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WB analysis of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)
human stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
WB analysis of rat stomach using 60130-1-Ig (same clone as 60130-1-PBS)
rat stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IF Staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IF Staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IF Staining of mouse stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.
IF Staining of mouse stomach using 60130-1-Ig (same clone as 60130-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

Gastrokine 1 Monoclonal antibody, PBS Only

Gastrokine 1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60130-1-PBS

Clone No.3C10E3

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60130-1-PBS targets Gastrokine 1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity	human, rat, pig, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag6091 Product name: Recombinant human GKN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 35-196 aa of BC059778 Sequence: NVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGWDSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQGKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEASLFFYSGTCYTTSVLWIVDISFCGDTVEN Predict reactive species
Full Name	gastrokine 1
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC059778
Gene Symbol	Gastrokine 1
Gene ID (NCBI)	56287
RRID	AB_2111459
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NS71
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Gastrokine 1 (GKN1), a stomach-speciﬁc protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.