Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat







Cat no : 66904-1-Ig


GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, Jurkat cells, K-562 cells, PC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66904-1-Ig targets Glucocorticoid receptor in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag28431
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC015610
Gene Symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).


Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IHC protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IF protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Antioxidants (Basel)

Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress

Authors - Wenji Ren

Am J Physiol Cell Physiol

Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.

Authors - Lisa M Cooper

Antioxidants (Basel)

Dim Blue Light at Night Induces Spatial Memory Impairment in Mice by Hippocampal Neuroinflammation and Oxidative Stress

Authors - Qi Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Shubham (Verified Customer) (04-15-2019)


  • Applications: Western Blot,
  • Cell Tissue Type: AC16
Glucocorticoid receptor Antibody Western Blot, validation ( dilution) in AC16 (Cat no:66904-1-Ig)