Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D9A9

Cat no : 66904-1-Ig

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66904-1-Ig targets Glucocorticoid receptor in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag28431
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC015610
Gene symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Protocols

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IHC protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IF protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Physiol Cell Physiol

Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.

Authors - Lisa M Cooper

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shubham (Verified Customer) (04-15-2019)

Good

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
Glucocorticoid receptor Antibody Western Blot, validation ( dilution) in AC16 (Cat no:66904-1-Ig)