Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow using 98269-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed.

• 

  FC experiment of mouse bone marrow using 98269-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98269-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98269-1-RR against Mouse Glycophorin A/CD235a were performed. The affinity constant is 19.4 nM.

Glycophorin A/CD235a Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98269-1-RR

Clone No.242129D10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

glycophorin A, Gypa, GPA, Glycophorin-A, CD235a

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98269-1-RR targets Glycophorin A/CD235a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: glycophorin A
• Calculated Molecular Weight: 18kDa
• GenBank Accession Number: NM_010369.3
• Gene Symbol: Gypa
• Gene ID (NCBI): 14934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P14220
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)