Tested Applications
Positive FC detected in | mouse bone marrow cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
98269-1-RR targets Glycophorin A/CD235a in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein Predict reactive species |
Full Name | glycophorin A |
Calculated Molecular Weight | 18kDa |
GenBank Accession Number | NM_010369.3 |
Gene Symbol | Gypa |
Gene ID (NCBI) | 14934 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P14220 |
Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
Protocols
Product Specific Protocols | |
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FC protocol for Glycophorin A/CD235a antibody 98269-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |