FcZero-rAb™ PE-Cyanine7 Anti-Human Granzyme B Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using PY7-FcA98070

  1x10^6 human PBMCs were intracellularly stained with 5 ul PE-Cyanine7 Anti-Human Granzyme B Rabbit RecAb (PY7-FcA98070, Clone: 240761G4) or 5 ul PE-Cyanine7 Rabbit IgG Isotype Control RecAb (PY7-FcA98136, Clone: 240953C9). Cells were then stained with CoraLite® Plus 405 Anti-Human CD8 (SK1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

Granzyme B FcZero-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. PY7-FcA98070

Clone No.240761G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

GZMB, 240761G4, Cathepsin G-like 1, CCPI, CGL 1

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PY7-FcA98070 targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Granzyme B fusion protein Eg0592
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• Conjugate: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 450-500 nm / 778 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10144
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). Granzyme B induces lamin B degradation in isolated nuclei less efficiently than Granzyme A (PMID:11331782).