FcZero-rAb® Biotin Anti-HA Tag Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-HA Tag Rabbit Recombinant Antibody (Biotin-FcA98447)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

HA Tag FcZero-rAb^® Recombinant Antibody for FC (Intra), ELISA

Cat No. Biotin-FcA98447 Copied!

Clone No. 251110A1

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Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

FC (Intra), ELISA

Synonyms

HA, YPYDVPDYA

• 

  FC experiment of Transfected HEK-293T using Biotin-FcA98447

  1x10^6 HEK-293T cells or transfected HEK-293T cells were intracellularly stained with 0.25 ug Biotin Anti-HA Tag Rabbit RecAb (Biotin-FcA98447, Clone: 251110A1) and Streptavidin-PE Conjugate (PE-PF00030). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98447 targets HA Tag in FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: HA Tag
• Calculated Molecular Weight: 1 kDa
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: HATAG
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.