FcZero-rAb® PE Anti-HA Tag Rabbit Recombinant Antibody

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• FcZero-rAb® PE Anti-HA Tag Rabbit Recombinant Antibody (PE-FcA98447)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

HA Tag FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. PE-FcA98447 Copied!

Clone No. 251110A1

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Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

FC (Intra)

Synonyms

HA, YPYDVPDYA

• 

  FC experiment of Transfected CHO using PE-FcA98447

  1x10^6 CHO cells or transfected CHO cells were intracellularly stained with 0.1 ug PE Anti-HA Tag Rabbit RecAb (PE-FcA98447, Clone: 251110A1). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Transfected CHO cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98447 targets HA Tag in FC (Intra) applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: HA Tag
• Calculated Molecular Weight: 1 kDa
• Gene ID (NCBI): 6
• RRID: AB_3749895
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: HATAG
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.