ChromoTek HA-Trap Agarose

Validation Data Gallery

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  The HA-Trap Agarose (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Agarose results in clean, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

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  Co-IP using HA-Trap Agarose followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

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  The HA-Trap Agarose was used to immunoprecipitate HA-PCNA and PCNA-HA proteins from transfected HEK293T cells. WB analysis was done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions of the IP using PCNA Monclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001). The HA-Trap is succesful in pulling down HA-tagged PCNA regardless of whether the tag is fused to the N- or C-terminal. Note: PCNA forms trimers, resulting in co-elution of endogenous PCNA with HA-tagged PCNA.

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  The HA-Trap Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution with HA-peptide (ap). Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms trimers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The ChromoTek HA-Trap Agarose consists of an anti-HA tag Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of HA-tagged proteins from cell extracts of various organisms such as humans, mice, dogs, yeast, and plants.

Cat No. ata

Publications(6)

Reviews (2)

Specificity

HA-Tag (sequence YPYDVPDYA)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Synonyms

Hemagglutinin tag, HA, HA-tag, HA-tag Trap Agarose

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Product Information

The ChromoTek HA-Trap Agarose consists of an anti-HA tag Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of HA-tagged proteins from cell extracts of various organisms such as humans, mice, dogs, yeast, and plants.

• Description: Immunoprecipitation of HA-tagged proteins with anti-HA-tag Nanobody conjugated to agarose beads.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
• Binding Capacity: 20 ug of recombinant HA-tagged protein (~30kDa) per 25 uL bead slurry
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions.
• Compatibility with mass spectrometry: The HA-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3094560
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
IP,CoIP	Plant Cell; Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.; Authors - Manikandan Kalidass
IP	Proc Natl Acad Sci U S A; MITD1 is a brain-specific interferon-inducible factor that inhibits flavivirus replication; Authors - Jim Zoladek
IP	Plant Cell Rep; A novel toolbox of GATEWAY-compatible vectors for rapid functional gene analysis in soybean composite plants; Authors - Joffrey Mejias
IP	Nat Commun; FGF receptor modulates planar cell polarity in the neuroectoderm via Vangl2 tyrosine phosphorylation; Authors - Ilya Chuykin
	bioRxiv; FGF receptor modulates planar cell polarity in the neuroectoderm via Vangl2 tyrosine phosphorylation; Authors - Ilya Chuykin
CoIP	Am J Hum Genet; Bi-allelic KICS2 mutations impair KICSTOR complex-mediated mTORC1 regulation, causing intellectual disability and epilepsy; Authors - Rebecca Buchert

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Giuliano (Verified Customer) (02-05-2026)

We were looking for an effective way of purifying HA-tagged proteins from B. subtilis under conditions where most of the commercially available agarose-coupled HA antibodies would perform poorly. After trying out ChromoTek HA-Trap Agarose and comparing it directly to what we were using so far, we were very happy with the result. The purification was very clean and the yield was noticeably high, all while fitting into our peculiar purification protocol with little to no adjustment. We highly recommend this product if, like us, you are dealing with issues in purity or yield, and especially contamination of the eluate due to the coupled antibody coming off the beads.

• Applications: Immunoprecipitation,Protein purification

FH

Hervé (Verified Customer) (04-09-2024)

IP very clean

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK