CoraLite® Plus 488-conjugated HAX1 Polyclonal antibody

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• CoraLite® Plus 488-conjugated HAX1 Polyclonal antibody (CL488-11266)

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• KD/KO Validated

HAX1 Polyclonal Antibody for IF/ICC

Cat No. CL488-11266

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

HAX 1, HAX-1, HCLS1-associated protein X-1, HCLSBP1, HS1-associating protein X-1

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  IF Staining of HeLa using CL488-11266

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HAX1 antibody (CL488-11266) at dilution of 1:200.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11266 targets HAX1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1792

  Product name: Recombinant human HAX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-279 aa of BC014314

  Sequence: MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR

• Full Name: HCLS1 associated protein X-1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014314
• Gene Symbol: HAX1
• Gene ID (NCBI): 10456
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00165
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.